86例儿童急性淋巴细胞白血病三色流式细胞术免疫分型研究

Immunophenotyping of eighty six children with acute lymphoblastic leukemia by three-color flow cytometry

目的 探讨儿童急性淋巴细胞白血病 (ALL)三色流式细胞术免疫分型的特点。方法 采用CD45 SSC双参数散点图设门方法进行三色流式细胞术免疫表型分析。结果 CD45 SSC双参数散点图设门方法与FSC SSC双参数散点图设门方法比较能将骨髓中的原始细胞与正常淋巴细胞、单核细胞及粒细胞明显分开 ,所得原始细胞比例与形态学分类接近 ,原始细胞免疫表型假阳性发生率低。 86例儿童ALL标本中 ,B ALL占 95 .3% ,T ALL占 2 .3%。Common ALL和Pro B ALL分别占B ALL的76 8%和 6 .1%。 5 7.0 %和 34 .9%的儿童ALL表达CD3 4 和髓系抗原 ,其中以Pro B ALL最常见。Pro B ALL中表达CD3 3 比CD1 3 多见 ,其它阶段B ALL中CD1 3 和CD3 3 的表达差异无显著性。结论 CD45 SSC双参数散点图设门方法排除了骨髓中正常细胞的干扰 ,免疫分型结果更可靠。儿童ALL有较高的CD3

Objectives To evaluate the specificity of three-color flow cytometry in childhood acute lymphoblastic leukemia (ALL) immunophenotyping. Methods Immunophenotyping was performed by three-color flow cytometry analysis using CD 45/SSC gating. Results The percentage of blasts was correlated better with leukemic cell count compared with that of FSC/SSC, and the false positive results were low. Among eighty six cases of ALL, 95.3% was B-ALL, in which common-ALL and Pro-B-ALL were 76.8% and 6.1%, respectively, and 2.3% was T-ALL. CD 34 + and myeloid-associated antigen expression were observed in 57.0% and 34.9% of the cases, respectively, among which Pro-B-ALL was the commonest. CD 33 was more commonly expressed than CD 13 in Pro-B-ALL cases, but no difference in the expression between these two antigens in other subtypes. Conclusion Gating of CD 45/SSC eliminated effection of normal cells to blasts in bone marrow, with which the immunophenotyping results were more reliable.