P53蛋白在喉癌细胞中的表达

Expression of P53 in laryngeal squamous carcinoma cell

①目的 对喉癌发生、发展的分子生物学机制进行初步的探讨。②方法 喉癌手术新鲜标本３０例，喉正常粘膜１６例为对照组。采用流式细胞术检测喉癌组织及正常粘膜的 Ｐ５３蛋白的含量，基因蛋白以荧光指数（ＦＩ）作为定量分析的指标，以 ＦＩ大于正常粘膜的ｘ＋２ｓ为阳性。③结果ａ.癌组织中Ｐ５３的阳性率高达９６ .７％，其ＦＩ均明显高于正常粘膜（Ｐ＜０．０１）。ｂ．临床Ⅲ、Ⅳ期癌组织Ｐ５３的ＦＩ明显高于Ⅰ、Ⅱ期（Ｐ＜０．０１）；Ｔ３、１４病变的Ｐ５３的ＦＩ明显高于Ｔ１、Ｔ２病变（Ｐ＜０. ０５）；淋巴结转移组的Ｐ５３的ＦＩ明显高于无淋巴结转移组（Ｐ＜０. ０１）。④结论 Ｐ５３蛋白在喉癌中的表达明显高于喉正常粘膜，且与临床分期、Ｔ分级及淋巴结转移呈正相关，与年龄、肿瘤大小和病理分级无关。

Objective To investigate the molecular biologic mechanism of the development and progression of la-ryngeal carcinoma. Methods 30 laryngeal carcinoma fresh samples were analyzed, and meanwhile 16 normal laryngeal mucosa samples were studied as controls. We measured the expression of P53 protein of all samples with the Flow Cy-tometer. Fluorescence Index (FI) was defined as the quantitative expression index of it. The FT value more than x± 2s of normal laryngeal mucosa should be considered positive expression. Results ①The positive rate of P53 protein expression in laryngeal carcinoma was 96.7% ,and its Fl significantly higher than that of normal mucosa (P<0. 01). ②The FI of P53 in the stage of Ⅲ Ⅵ and T3,T4 were significantly higher than those in stage of Ⅰ -Ⅱ and T1,T2(P <0.01, P<0. 05). The FI of P53 protein in metastatic group was significantly higher than that in non-metastatic group (P<0. 01). Conclution The expression of P53 protein in laryngeal carcinoma was significantly higher than that in normal mucosa and correlated with the clinical stage, T stage and metastasis positively.