银杏叶提取物对培养的海马神经元内谷氨酸诱导钙信号的影响

Effect of extract in leaves of Ginkgo Biloba on calcium signal of cultivated neurons induced by glutamate

目的检测银杏叶提取物(ginkgobilobaextract,GBE)对谷氨酸升高培养的海马神经元内游离钙离子浓度(犤Ca2+犦i)的影响和特征。方法实验设计分3组(n=26),向细胞吹药各20s:①组1×10-5mol/L谷氨酸,②组同时1×10-5mol/L谷氨酸。25μg/mlGBE,③组即在②组回到基线后给1×10-5mol/L谷氨酸。结果①组犤Ca2+犦i明显升高,②组犤Ca2+犦i的变化明显变小,其峰值明显下降,且Phase1上升速度减慢,Phase2的时间也有所缩短,二相之间的平台期相对延长,③组出现与①组中相似的钙震荡表现,但Phase1和Phase2的时间均缩短,犤Ca2+犦i的变化稍低。结论GBE通过影响NMDA受体的活动。快速抑制谷氨酸诱导大鼠海马神经元内游离钙浓度升高。

Objective To determine whether the extract of leaves of Ginkgo biloba extrac(GBE) can inhibits the increase of intercellar free calcium(i) induced by glutamate (Glu).Method There are three groups, ①give 1×10-5 mol/L glutamate for 20 seconds; ②give 1×10-5 mol/L glutamate and 25 μg/ml GBE at the same time for 20 seconds; ③give 1×10-5 mol/L glutamate after i of group back to the baseline.Result GBE can obviously inhibits the increase of i induced by Glu and it can also effect the time of calcium oscillation, it can slow the increase of i,fast the decrease of i.Conclusion EGb effecting the active of NMDA receptors and prevent neurons from Glu neurotoxicity through reduction of the rise in i.