白介素-10对TNF-α介导血管平滑肌细胞增殖的影响

Effects of Recombinant Human Interleukin-10 on Rat Vascular Smooth Muscle Cells Proliferation by Tumor Necrosis Factor-α

观察重组人白介素 10 (rhIL_10 )对肿瘤坏死因子 (TNF_α)刺激的离体大鼠胸主动脉血管平滑肌细胞增殖的影响。体外培养SD大鼠胸主动脉血管平滑肌细胞 ,采用MTS PES法确定血管平滑肌细胞的增殖状态。结果显示 ,TNF_α对血管平滑肌细胞增殖具有明显的刺激作用。rhIL_10单独应用对血管平滑肌细胞生长没有影响。在TNF_α刺激下 ,低至 10ng mL的rhIL_10可明显抑制血管平滑肌细胞的生长 (P <0 0 5 )。

The purpose of this study was to determine the effects of recombinant human interleukin-10 (rhIL-10) on rat vascular smooth muscle cell proliferation stimulated by tumor necrosis factor-α(TNF-α). Rat aortic vascular smooth muscle cells(VSMCs) were cultured and treated with recombinant human interleukin-10(rhIL-10)with or without tumor necrosis factor-α (TNF-α). Proliferation of VSMCs was quantified by colorimetric assay. The results indicated that TNF-α stimulated VSMCs proliferation and rhIL-10 alone had no effect on VSMCs growth. With TNF-α stimulation, rhIL-10,at dose as low as 10 ng/mL, inhibited VSMCs growth \%(P<0.05).\%