羧乙基锗倍半氧化物对大鼠肺缺血再灌注损伤的保护作用

The protective efects of carboxyethyi-germanium squioxide on lung ischeia-reperfusion injury in rats

采用 Wistar大鼠原位肺缺血再灌注损伤模型 ,测定再灌注后左肺动脉压、左肺湿干比质量值 ,肺组织中丙二醛 (MDA)含量 ,超氧化物歧化酶 (SOD)及 ATPase活性。发现预防性静脉注射羧乙基锗倍半氧化物 CGS(5 0 mg/kg)后 ,与对照组和假手术组相比较 ,可显著降低再灌注肺组织中 MDA、左肺动脉压及肺湿干质量比值(F =6 .37,q =8.98,P<0 .0 1;F=3.85 ,q=4.2 1,P<0 .0 5 ;F=4.0 6 ,q=5 .38,P<0 .0 0 1) ;明显提高肺组织中SOD及 ATPase活性 (F=6 .37,q=10 .2 1,P<0 .0 0 1;F=6 .37,q=8.6 4,P<0 .0 5 )。认为 CGS对大鼠实验性肺缺血再灌注损伤具有保护作用。

In order to observe the effects of carboxyethyl-germandium sesquioxide(CGS)on lung ischemia-reperfusion,the experimental model was established by ligaturing left lung hilum for 30 minutes followed by reperfusing for 60 minutes in the rats The left pulmonary artery pressure,left lung Mw/Md,level of MDA,activity of SOD and ATPase were measured The results showed after CGS (50mg/kg) was intravenous injected,the level of MDA decreased, activity of SOD and ATPase in lung of rats were increased respectively (F=6 37,q=8 98,P<0 01;F=3 85,q=4,21,P<0 05;F=4 06,q=5,38,P<0 001;F=6 37,q=10 21,P<0 001;F=6 37,q=8,64,P<0 05) This suggest that CGS might have a protective effect on the damage of lung ischemia-reperfusion in rats