尿中乙型肝炎病毒DNA检测对小儿乙肝相关性肾炎诊断价值研究

Study on correlation of detection of HBV-DNA in urine to diagnosis of children with hepatitis B virus associated glomerulonephritis

目的 探讨尿中乙肝病毒DNA(HBV DNA)的检测对诊断小儿乙肝病毒相关性肾炎 (HBV GN)的意义。方法 应用聚合酶链式反应 (PCR)、免疫荧光 (IF)及原位杂交法 (ISH)检测患儿血清和尿中HBV DNA及肾活检石蜡包埋组织切片中HBV抗原与HBV DNA的存在状况。结果 32例患儿经血清HBV DNA与肾组织中HBV抗原检测 ,符合HBV GN诊断的 19例 ,其中 11例经ISH检测发现肾活检石蜡包埋组织切片中HBV DNA阳性 ,13例尿HBV DNA阳性 ,阳性率分别为 5 7 9%、6 8 5 %。结论 检测肾小球疾病患儿尿中HBV DNA可协助HBV

Objective To study the significance of detection of HBV-DNA in urine in diagnosis of hepatitis B virus associated glomerulonephritis(HBV-GN).Methods To detect respectively HBV-DNA in serum and in urine,and HBV-Ag and HBV-DNA in paraffin-embedded renal biopsy tissue by polymerase chain reaction(PCR),immuno fluorescence(IF),and in situ hybridization(ISH).Results 19 from 32 objects with HBV-GN were diagnosed by detecting HBV-DNA in serum and HBV-Ag in paraffin-embedded renal biopsy tissue . HBV-DNA were positive in urine in 13 out of 19 objects and in 11 out of 19 objects in paraffin-embedded renal biopsy tissue, the positive rate was 68.5% and 57.9% respectively.Conclusion Detecting HBV-DNA in urine for patients with nephrosis may be helpful in diagnosis of HBV-GN.