子宫内膜癌微血管密度及血管内皮生长因子表达的研究

Study of microvessel density and the expression of vascular endothelial growth factor in endometrial carcinoma

目的 探讨子宫内膜癌血管内皮生长因子 (VEGF)的表达 ,微血管生成及其与临床病理参数的关系。方法 采用S P免疫组化染色法检测 50例子宫内膜癌及 1 0例正常子宫内膜中VEGF的表达及计数微血管密度 (MVD)。结果 子宫内膜癌中VEGF表达阳性率 (92 % ) ,MVD(46 .0± 4 .5)显著高于正常子宫内膜的表达率 (30 % ,MVD1 4 .0± 1 .7,P <0 .0 1 )。 43例子宫内膜癌浸润深度 <1 / 2肌层 ,其VEGF表达率为 90 .7% ,7例肌层浸润深度 >1 / 2 ,VEGF全部阳性表达 ,经统计学处理差别无意义 ,肿瘤浸润深度 >1 / 2肌层组MVD(69.9± 1 6 .8)显著高于浸润深度 <1 / 2肌层组 (42 .8± 4 .4) ,差别有统计学意义 (P <0 .0 5)。VEGF表达率 ,MVD与其它临床病理因素不相关。结论 VEGF ,MVD在子宫内膜癌中高表达 ,说明VEGF ,MVD与子宫内膜癌的发生有关。

Objective To investigate the expression of vascular endothelial growth factor (VEGF) and angiogenesis in endometrial carcinoma and the relationship with clinicopathologic factors.Methods S P immunohistochemical method was used to detect VEGF expression and MVD in 50 cases of endometrial carcinoma and 10 cases of normal endometrium. Results The positive rate of VEGF(92%) and MVD in endometrial carcinoma (46.0±4.5) were both higher that those in normal endometrium (30%,14.0±1.7 respectively).The difference was significant ( P <0.01). There were 43 cases of endometrial carcinoma which have myometrial invasion of one-half or less, the positive rate of VEGF was 90.7%. Seven cases of myometrial invasion of more than one half were all positive of VEGF. The difference was not significant ( P >0.05). MVD of myometrial invasion of one half or more group (69.9±16.8) was more than that of less than one-half group(42.8±4.4).The difference was significant ( P <0.05). No correlation was examined among the expression of VEGF, MVD and other clinicopathologic factors in endometrial carcinoma. Conclusion Over expression of VEGF and MVD is found in tissues of endometrial carcinoma. MVD may be one of prognostic indicators of endometrial carcinoma.