中国北方汉族人微粒体环氧化物水解酶基因多态性与慢性阻塞性肺疾病易感性的关系

Microsomal epoxide hydrolase gene polymorphism and susceptibility to chronic obstructive pulmonary disease in Han nationality of North China

目的 探讨中国北方汉族人微粒体环氧化物水解酶基因多态性与慢性阻塞性肺疾病(COPD)易感性的关系。方法 应用聚合酶链反应限制性片段长度多态性法 ,检测微粒体环氧化物水解酶基因型在 5 5例中国北方汉族人吸烟COPD患者和 5 2例健康吸烟者中频率的分布。结果 5 5例吸烟COPD患者微粒体环氧化物水解酶外显子 3的同源野生型、杂合型、同源突变型频率分布分别为2 7.3%(15 / 5 5 )、2 7.3%(15 / 5 5 )、45 .5 %(2 5 / 5 5 ) ,外显子 4的同源野生型、杂合型、同源突变型频率分布分别为 72 .7%(4 0 / 5 5 )、18.2 %(10 / 5 5 )、9.1%(5 / 5 5 ) ,与 5 2例健康吸烟者的分布比较差异无显著性。结论 微粒体环氧化物水解酶基因与中国北方汉族人COPD易感性无关。

Objective We investigated whether polymorphism in gene for microsomal epoxide hydrolase (mEH) has any bearing on individual susceptibility to the development of chronic obstructive pulmonary disease. Method The genotypes of 55 patients with COPD and 52 healthy smoking control subjects were tested with polymerase chain reaction followed by restriction fragment length polymorphism for mEH gene. Result The frequency of polymorphic genotypes of mEH showed no difference between the COPD group and the control group. In COPD group mEH exon 3 homozygous wild-type, heterozygote and homozygous mutant was 27.3%, 27.3% and 45.5% respectively and exon 4 homozygous wild-type, heterozygote and homozygous mutant was 72.7%, 18.2% and 9.1% respectively. Conclusion Genetic polymorphism in mEH is not associated with development of COPD in Han nationality of North China.