大鼠创伤后应激障碍模型的建立及其海马糖皮质激素受体的变化

Establishment of PTSD model in rats and expression of glucocorticoid receptor in the hippocampi

目的 建立较理想的创伤后应激障碍 (PTSD)动物模型 ,并初步探讨脑组织糖皮质激素受体 (GR)变化规律。方法 采用频率 2 5Hz、波宽 1ms、串长 10s、串隔 7min、强度 10 0 μA的恒流、单脉冲电流 ,反复刺激大鼠海马建立PTSD模型 ,观察实验动物情感行为改变 ;并利用Westernblot法检测脑组织GR表达。结果 通过反复惊厥阈下电刺激海马 ,成功诱发了实验动物较长时程的明显活动习性改变、警觉水平增高、惊恐行为、环境适应能力下降、躲藏逃避反应等多种PTSD样情感行为障碍 ;电刺激停止后 1周内海马GR表达明显增高。结论 海马惊厥阈下电刺激可基本满足PTSD主要临床表现 。

Objective To establish a relatively ideal animal model of posttraumatic stress disorder (PTSD), and observe the expression of glucocorticoid receptor (GR) in brain tissues of the model. Methods The hippocampus of Wistar rat was repeatedly stimulated with single pulse constant pulsating current of 100 μA,16 Hz in intratrain frequency, 1 ms in pulsating duration, 10 s in train duration, and with interstimulus interval of 7 min, 8 times per day for 5 d. The emotional behaviors of experimental rats were obsemed, and the GR expression was detected with Western blotting. Results The experimental rats displayed a long term significant abnormalities of PTSD like emotional behaviors, such anxiety like behariors as substantial changes in behavioral habits, hyperarousal, frightenedly hypersensitive reactions. And the GR expression in the hipocampus was increased remarkably. Conclusion The animal model of subconvulsive stimulating to hippocampus fulfills most characteristics in PTSD, and the constantly increased GR expression may play an important role in the long term persistent neuropsychological sequelae of PTSD.