大鼠失血性休克复苏后胃损害观察

Gastric injury posterior to hemorrhagic shock resuscitation in rats

目的 观察大鼠失血性休克复苏后的胃损害。方法 10 8只Wistar大鼠随机分为假休克 (SS)组和失血性休克复苏(HS)组 ,同位素标记生物微球法测量胃肠道血流量 ,同时测定胃粘膜内pH(pHi)、胃排空、胃粘膜 ,丙二醛 (MDA)含量和Na+ K+ AT Pase活性 ,并观察胃粘膜组织学和超微结构变化 ,以及胃粘膜Fos、Jun的免疫组化染色。结果 HS组各时相点胃血流量和pHi均显著下降 ,胃排空减慢 ,胃粘膜MDA含量显著升高 ,Na+ K+ ATPase活性明显受抑。HS组胃粘膜有明显的病理损害 ,胃粘膜Fos和Jun免疫反应性校正面积及免疫反应阳性细胞数显著增加。结论 在失血性休克复苏后 ,胃血流量下降 ,伴随胃排空延迟 ,并存在严重的缺血 再灌注损害 。

Objective To investigate the gastric lesion after resuscitation due to hemorrhagic shock.Methods 108 Wistar rats were randomly divided into sham shock (SS)group and hemorrhagic shock resuscitation (HS) group. The volume of gastric blood flow was determined by biological microglobulin isotope labelling methol. Gastric PHi, gastric emptying, the volume of gastric mucosa MDA, and Na + -K + - ATPase activity of gastric mucosa were determined. Pathological and ultrastructural changes of gastric mucosa were observed. Fos and Jun immunohistochemical staining of gastric mucosa were perfomed. Results After hemorrhagic shock resuscitation,the volume of gastric blood flow and gastric pHi and decreased significantly, gastric emptying retarded obviously, and the reactivity of Na + K + ATPase of gastric mucosa was inhibited. However, the volume of MDA in gastric mucosa increased. Meanwhile, there were significant pathological changes of gastric mucosa. In HS group, and the immunoreactivity expression area of gastric mucous membrane Fos and Jun were significantlyenlarged with a significant increase in positively reactive cells. Conclusion After hemorrhagic shock resuscitation, the volume of gastric blood flow decreases, accompanied with retarded gastric emptying. And there is severe gastric ischemia/reperfusion damage which is believed correlated with the development of other pathological changes of gastric mucosa.