γ-干扰素抑制人类口腔黏膜成纤维细胞生长的实验研究

Effects of interferon-gamma on human oral fibroblasts in vitro

目的 检测γ 干扰素是否具有直接抑制人类口腔黏膜成纤维细胞生长的作用。方法 将γ 干扰素作用于体外培养的口腔黏膜成纤维细胞 ,并用MTT比色法检测其对成纤维细胞增殖的影响。结果 γ 干扰素对成纤维细胞有明显的抑制作用。结论 γ 干扰素可能是创伤愈合等纤维反应的生理调节因子 。

Objective To study whether IFN γ can directly inhibit the proliferation of oral human fibroblasts. Methods The fibroblasts were obtained from human normal oral mucosa and cultured in vitro.Then the cell proliferation of fibroblasts incubated with or without IFN γ in the presence of 10% fetal calf serum for 48 h at 37 ℃in 5% CO 2 were monitered by use of thiazolyl blue (MTT) assay. Results IFN γ inhibited fibroblast proliferation in a concentration dependent manner. Conclusions IFN γ can directly inhibit human oral fibroblasts proliferation. It may be a promising pharmaceutical agent for the reduction of scarring after surgery and for the treatment of oral submucous fibrosis.