小剂量MIP-1α联合IL-8体外对人骨髓造血细胞集落形成的影响

Effect of low dose MIP-1α combined IL-8 on clone formation of hurman lone marrow hematopoietic cells

目的 了解小剂量巨噬细胞炎症蛋白 1α(Macrophageinflammatoryprotein αMIP 1α)联合白细胞介素 8(Interleukin 8,IL 8)对人骨髓造血细胞集落形成的影响。方法 取人骨髓 ,根据随机单位组方差分析设计 ,将每例标本分为空白组 (不加MIP 1α和IL 8) ;10ng ml联合组 (加入 10ng ml的MIP 1α和IL 8) ;1ng ml联合组 (加入 1ng ml的MIP 1α和IL 8) ;单用MIP 1α组 (加入 2 0ng ml的MIP 1α) ;单用IL 8组 (加入 2 0ng ml的IL 8)。建立半固体培养体系 ,一定时间后 ,观察集落粒单细胞集落形成单位 (CFU GM)、红细胞集落形成单位 (CFU E)和混合集落 (CFU Mix)的形成情况。结果 1ng ml的浓度下 ,相同浓度的MIP 1α与IL 8联合使用对人骨髓造血细胞集落的形成没有明显的抑制作用 ;而在 10ng ml的浓度下 ,两者联合使用则能明显地抑制人骨髓造血细胞集落的形成。两种因子单独使用 ,未见明显的集落形成抑制作用。结论 10ng mlMIP 1α与IL

Objective To study the effect of low dose Macrophage inflamatory prolein α (MIP 1α) combined with IL 8 on clone formation of human bone marrow hematopoietic cells. Methods Bone marrow hematopoietic cells were divided into 5 groups: control group (with no MIP α or IL 8) , 10 mg/ml combination group (with 10 ng/ml MIP lx and 10 ng/ml IL 8), 1 mg/ml combination group (with 1 ng/ml MIP lα and 1 ng/ml IL 8) ,MIP lα group (with 20 ng/ml MIP), and IL 8 group (with 20 ng/ml IL 8). Then these cells were cultivated in 0 9% methyl cellulose system. Several days later, count the clones of CFU GM, CFU E and CFU Mix of each group. Results At the concentration of 1 ng/ml, combination of MIP 1α and IL 8 didn't inhibit the clone formation but the 10 ng/ml combination group did obviously. Neither MIP 1α nor IL 8 alone did it in our study. Conclusion Combination of MIP 1α and IL 8 both at 10 ng/ml can coordinate each other to inhibit the clone formation of human bone marrow hematopoietic cells.