川芎嗪对大鼠缺血再灌注视网膜SOD,MD和NO水平及细胞凋亡的影响

Ligustrazine on rat retinal cell apoptosis and on the levels of SOD, MDA and NO after retina ischemia reperfusion injury

目的 :观察川芎嗪对大鼠视网膜缺血再灌注后视网膜超氧化物歧化酶 (superoxidedismutase ,SOD)、丙二醛 (malondialdehyde ,MDA)、一氧化氮 (nitricoxide ,NO)水平及视网膜细胞凋亡的影响。方法 :采用大鼠视网膜压力缺血再灌注模型 ,分光光度法测定SOD、MDA和NO ,琼脂糖凝胶电泳分析DNA断裂。结果 :视网膜缺血 6 0min再灌注后SOD水平下降 ,而MDA和NO水平则升高 ;视网膜缺血 6 0min再灌注 12h ,提取DNA进行琼脂糖凝胶电泳可见凋亡样DNA断裂 (ApoptoticDNAfragmentation)。川芎嗪能显著对抗视网膜缺血再灌注时视网膜SOD水平的下降、MDA和NO水平的升高 ;同时能阻断大鼠视网膜缺血再灌注 12h后视网膜细胞DNA凋亡样断裂。结论

Objective:To study the effect of ligustrazine on the levels of superoxide dismutase(SOD),malondialdehyde(MDA)and nitric oxide(NO);and the retinal apoptosis after rat retina ischemia reperfusion injury. Methods:Retinal ischemia was induced in adult rats by raising the intraocular pressure to 110 mmHg for 60 min.The levels of SOD,MDA and NO were measured by photometry.DNA fragmentation was valued by agarose gel electrophoresis. Results:The level of SOD decreased,that of MDA and NO both increased in rat ischemia reperfusion retina.Those changes were all blocked by ligustrazine.After reperfusion 12h,retinal apoptotic DNA fragmentation was detected and the administration of ligustrazine inhibited the apoptotic DNA fragmentation. Conclusions:The apoptosis induced by ischemia reperfusion injury can be blocked by ligustrazine that might be through blocking the generation of free radicals and elevating the ability of antioxidisity in rat retina.