先兆子痫患者外周血单个核细胞培养上清液白细胞介素2和γ干扰素的检测

Detection of IL-2 and IFNγ in the Cell Culture Supernatants of Peripheral Blood Mononuclear Cell From Patients With Preeclampsia

目的 :探讨Th1型细胞因子在先兆子痫中变化。方法 :采用ELISA方法检测 15例先兆子痫患者及 15例正常孕妇外周血单个核细胞 (peripheralbloodmononucleorcell,PBMC)培养上清液中白细胞介素 2 (IL 2 )和γ干扰素 (IFN γ)产生水平。结果 :先兆子痫患者IL 2 (2 79.0± 12 3.7pg/ml)及IFNγ(46 6 .9± 16 5 .4 ) pg/ml水平升高差异非常显著 ,均为P <0 .0 0 1;两者成显著正相关 ,r为 0 .6 2 6 ,P <0 .0 1;且IL 2与IFNγ产生水平与平均动脉压呈显著正相关 ,r为 0 .6 19及 0 .6 5 9,均为P <0 .0 1。结论 :先兆子痫患者Th1型细胞因子分泌亢进 。

Objective: The paper was to study the change of Th1 type cytokines in preeclampsia.Methods: Fifteen patients with preeclampsia and 15 healthy pregnant women were selected randomly. Interleukine 2 (IL 2) and interferin γ (IFNγ), levels in cell culture supernatants of PBMC were detected by ELISA.Results: IL 2 and IFNγ levels of preeclampsia group were increased significantly compared with those of control group (P<0 001). IL 2 level was positively correlated with IFNγ level. (r=0.626,P<0 01);IL 2 and IFNγ levels were significantly correlated with MAP(r= 0.619 and r=0.659,P<0 01).Conclusion: There were increased secretion of TH1 cytokines in preeclampsia, and the level of TH1 cytokines were correlated with MAP.