表阿霉素诱导人肝癌SMMC-7721细胞凋亡的实验研究

The experimental research of the induction of apoptosis in human hepatocelluar cancer cell line SMMC-7721 by Pharmorubicin.

目的 进一步探讨表阿霉素治疗原发性肝癌的作用机理。方法 应用DNA电泳、电镜、流式细胞仪分析技术 ,观察表阿霉素诱导人肝癌SMMC 772 1细胞凋亡模式。结果 0 1μg/ml表阿霉素作用细胞 12、2 4、36、48小时及 0 1、1 0、10 0、10 0 μg/ml表阿霉素作用细胞 2 4小时均可出现细胞凋亡现象 ,其诱导凋亡效率与剂量、时间呈正相关。结论 表阿霉素可诱导人肝癌SMMC 772

Objective To further investigate the mechanism of the treatment of Pharmorubicin on human hepatocelluar cancer.Methods The induction of apoptosis by pharmorubicin in human hepatocelluar cell line SMMC-7721 was investigated by applying DNA electrophoresis,electron microscopy and flow cytometry analysis techniques.Results Apoptotic phenomena were present in cells after treatment with Pharmorubicin at the final concentration of 10μg/ml for 12,24,36,48 hours and after treatment for 24 hours with Pharmorubicin at the final concentration of 0.1,1.0,10,100μg/ml respectively.Efficiency of apoptosis was enhanced with the increasing of dose and time.Conclusion Pharmorubicin might have anti- tumor effect by the induction of apoptosis in human hepatocelluar SMMC-7721 cells.