大鼠实验性视网膜缺血组织中线粒体的体积密度定量及动态研究

A Quantitative and Dynamic on Mitochondrion Volume density in Ischemic Tissues of Rat's Experimental Retina

目的 研究视网膜缺血组织中线粒体的体积密度参数变化特点规律。方法 给 SD大鼠球后注射去甲肾上腺素 ,建造视网膜缺血的动物模型 ,用电镜和图像分析仪等方法 ,对缺血视网膜组织中线粒体的体积密度参数动态改变进行了体视学定量分析。结果 大鼠球后注射去甲肾上腺素后 ,视网膜内节、外网状层及内网状层在缺血状态下 ,线粒体空泡的体积密度增高 ,尤以内网状层表现最明显 ,并随着时间变化有显著性差异。呈现缺血→损伤→修复的过程。结论 球后注射去甲肾上腺素后 ,视网膜线粒体的体积密度发生缺血性改变 ;缺血视网膜组织不同部位的损伤 ,其线粒性的体积密度参数改变不同 ;线粒体对缺血极为敏感 ,反应最强烈。

Objective Study the parametric changes and rules of the mitochondrion volume density in retina's ischemic tissues.Methods Use SD rat postbulbar injection of noradrenalin to build up the animal model with retinal ischemia and carry out a stereologic quantitative analysis of the parameter's dynamic changes of mitochondrion volume density in retina's ischemic tissues through electromicroscope technique,image pattern analysis,etc.Results After taking the postbulbar injection of noradrenalin,and as the internal segment,as well as the external reticular layers,of retina remains ischemic,the volume density of the mitochondrion vaculation were increased,especially the internal reticular layer's changes,and varies significantly in process of time.The entire development progress of ischemia to injury to recovery.Conclusion After taking the postbulbar injection of noradrenalin,ischemic changes take place in retina's mitochondrion volume density with parameters changing in accordance with injuries in different parts of ischemic retinal tissues.Mitochondrion is quite sensitive to ischemia and has strong reaction.