组织工程血管种子细胞培养的实验研究

CULTURE OF SEED CELL FOR VASCULAR TISSUE ENGINEERING

目的 :为血管组织工程的实验研究提供细胞来源。 方法 :分别采用酶消化法和组织块法培养血管内皮细胞和血管平滑肌细胞 ,并进行两种细胞的传代、纯化、鉴定以及形态学观察。 结果 :培养的两种细胞均符合血管内皮细胞和血管平滑肌细胞的形态特征。 结论

Objective:To provide cells resource for the experimental study on vascular tissue engineering Methods:Vascluar endothelial cells and smooth muscle cells are respectively isolated from rabbit thoracic aorta by enzyme digestion and tissue plant methods,subcultured,purified and identified,etc Phase-contrast and electron microscopy was used to analyze the cells morphological characteristics Results:The morphological characteristics of two kinds of cells are accord with those of vascular endothelial cells and smooth muscle cells Conclusion:The cultured cells can meet the experimental expectations on vascular tissue engineering