青蒿琥酯对人宫颈癌HeLa细胞的乏氧杀伤和辐射增敏作用初探

Primary Study of Effect of Hypoxic Killing and Radiation Sensitivity of Artemisinin on Human Cervical Cancer Cell

目的:观察青蒿琥酯对人宫颈癌HeLa细胞的乏氧杀伤及辐射增敏作用的影响。方法:采用Mosman'sMTT细胞增殖检测法。结果:青蒿琥酯(Artemisinin,Art)能够抑制人宫颈癌HeLa细胞的生长,其半数抑制浓度(IC50)为37μg/ml。在乏氧条件下,青蒿琥酯对HeLa细胞的IC50为30μg/ml。在本实验条件下,咪嗦哒唑(MISO)的辐射增敏比(SER)为1.39,而青蒿琥酯在10μg/ml的SER为1.32,在30μg/ml的SER为2.00。结论:青蒿琥酯具有一定的辐射增敏作用。其辐射增敏作用是否与其结构有关尚待深入研究。

Objectives: To investigate the effect of hypoxic killing and radiation sensitivity of artemisinin on human cervical carcinoma cell line HeLa cell. Methods: The Mosmans MTT method was used to determine the cellular proliferation. Results: Artemisinin (Art) had inhibitory effect on HeLa cell, the 50% inhibitory concentration (IC50) was 37 μg/ml, the 50% inhibitory rate of artemisinin on the HeLa cell in the case of hypoxic was at the concentration of 30 μg/ml. The radiation sensitivity enhancement ratio (SER) of the radiosensitivity agent Misonidazone (MISO) was about 1.39, and that of arteminsinin was 1.32 or 2.00 when the concentration of Art was 10 μg/ml or 30 μg/ml,respectively. Conclusions: Artemisinin has some radiosensitivity effect, the relationship between the chemical structure and radiosensitivity effects of Art should be further studied.