海马不同区域缺血时损伤和hsp70mRNA表达差异

Injury and expression of hsp70mRNA in different areas of rat hippocampus after ischemia

目的 :探讨缺血再灌注后鼠脑海马CA1 区选择性易损伤和迟发神经元死亡现象,以及hsp70mRNA差异表达的可能机制。方法 :观察鼠前脑缺血再灌注时 ,海马CA1 区、CA3 区及齿状回锥体细胞在组织学变化 ;以及原位杂交技术检测各区hsp70mRNA的诱导表达。结果:缺血再灌注7d后海马CA1 区锥体细胞缺失明显,而CA3 区及齿状回未见明显形态学变化。CA1 区hsp70mRNA的诱导较其余两区迟,而持续时间较长。结论:在缺血早期加强或在缺血后期抑制hsp70mRNA的诱导,有可能保护CA1 区锥体细胞。

Objective: To clarify selective vulnerable (SV) and delayed neuronal death (DND) in CA 1 sector after ischemia and reperfusion, and the mechanism of different hsp70mRNA expression in different sectors of rat hippocampus. Methods: A 10-min forebrain ischemic model in rats was established, the histology change of neurons were assessed in CA 1, CA 3 sectors and dentate gyrus, and hsp70mRNA expression was detected by method of in situ hybridization. Results: Histopathological analysis revealed that transient ischemia produced obvious neurons loss at 7 days recirculation in CA 1 sector, however there were not evident neuronal damage in CA 3 sector and dentate gyrus. Hybridization of hsp70mRNA in CA 1 sector cells after ischemia presented later, but prolonged longer than other sectors. Conclusion: The present study indicated that enhanced hsp70mRNA expression in early phase,or reduced the expression of hsp70mRNA in late phase after ischemia might prevent cells from DND.