骨折愈合刺激素对体外培养成骨细胞的影响

The effects of fracture-healing stimulin on osteoblasts in vitro

目的 观察骨折愈合刺激素对体外培养成骨细胞的作用。方法 在新生SD大鼠头颅骨次代成骨细胞 (OB2 )培养液中分别加入不同浓度 (2× 10 2 U/L～ 2× 10 4U/L)骨折愈合刺激素 ,观察OB2的增殖功能 (用波长 5 70nm处OD值表示 ) ,取药物敏感浓度 (2× 10 2 U/L)分别观察分化功能〔用碱性磷酸酶 (ALP)活性表示〕和矿化功能 (用矿化结节数量 /视野表示 )。结果 OB2 增殖功能 (OD值 )实验组为 0 336± 0 0 73～ 0 35 9± 0 0 5 1,对照组为 0 347± 0 0 35 ;OB2 分化功能〔ALP(U/g蛋白质 )活性〕实验组为 83± 9,对照组为 81± 4 ;OB2 矿化结节数量 /视野 (个 )实验组为 6 0± 1 82 6 ,对照组为1 5± 1 0。结论 骨折愈合刺激素各浓度对OB2 的增殖和分化功能均无明显作用 (P >0 0 5 ) ,而对其矿化功能具有明显的刺激作用 (P <0 0 1)。

Objective To observe the effects of the fracture healing stimulin, a fracture healing increasing drug from staphylococcus aurous on osteoblasts in vitro . Methods The culture medium with different concentrations (2×10 2 U/L～2×10 4 U/L) of the fracture healing stimulin and the second generation of osteoblasts (OB 2) from the skull of newborn SD rat were mixed for the observation about the proliferation (OD value at 570 nm), for the observation respectively about the differentiation (ALP activity) and the mineralization (mineralized nodes/field of vision) of the OB 2 with the sensitive concertrations(2×10 2 U/L). Results The OD value of experimental group was 0 336±0 073～0 359±0 051, and that of control group 0 347±0 035; the ALP activity of experimental group was (83±9) U/g proteins, and that of control group (81±4) U/g proteins and the mineralized nodes of experimental group was 6 0±1 826, and that of control group 1 5±1 0. Conclusion The proliferation and differentiation of the OB 2 were not obviously influenced ( P >0 05) by the fracture healing stimulin, but the mineralization was remarkably increased( P <0 01).