络通对糖尿病大鼠肾脏的保护作用及机理研究

Efficacy and Mechanism of Luotong on Diabetic Nephropathy in Strptozotocin-induced Diabetic Rats

目的 :探讨络通对糖尿病大鼠肾功能的作用及其可能机理。方法 :4 0只SD大鼠随机分成正常对照组、模型组与络通组。络通粉末 (4g kg·d-1)混入普通饲料给药 ;实验间期 180d。实验期间每月测 1次血糖 ,实验第 90天、180天分别测 2 4h尿蛋白量 ,尿微量白蛋白及尿NAG并经尾静脉取血测血清尿素氮(BUN)、肌酐 (Scr)。实验结束时颈动脉取血测血浆一氧化氮 (NO)、丙二醛 (MDA)、全血谷胱甘肽过氧化物酶(GSH PX)、甘油三脂 (TG)等。结果 :3个月时络通组尿微量白蛋白与尿肌酐的比值较模型组有明显减少 (P<0 0 5 )。 6个月时络通组 2 4h尿蛋白已显著低于模型组 (P <0 0 0 1) ,尿NAG、TG、MDA等也明显改善 (P <0 0 5 )。结论 :络通虽不能完全保护或阻止DN的发生发展 ,但可延缓或减轻DM大鼠蛋白尿的出现。这可能与络通能减轻氧自由基损伤 ,提高机体抗脂质过氧化作用等有关。

Objective: To evaluate the protective effect and mechanism of luotong on streptozotocin induced diabetic nephropathy. Methods\ Three groups of male sprague dawley rats were studied:normal control rats, streptozotocin diabetic rats and diabetic rats treated with luotong (4/kg·d -1 ). Serum creatinine, urea nitrogen, urinary albumin excretion rate and NAG as well as urinary microalbumin excretion rate, were observed at 3,6 months of treatment. At six months after treatment, all were animal were killed, triglyceride, carbonmonoxide, malondialdehyde, as well as glutathione peroxidase (GSH PX) were observed. Results\ At three months, the preventive group exhibited statistically significant decrease in the urinary microalbumin excretion rate, comparied to model groups. At six months, luotong treated diabetic rats caused a significant decrease in the urinary albumin excretion, the urinary albumin excretion, the urinary NAG, serum triglyceride and MDA. Conclusions\ Early treatment of luotong may be helpful in delaying or possibly preventing the dibetic nephropathy, and this effect may be partly achieved by lipid lowing and decreasing the lesions of oxygen free radical.