铁树叶提取液对白血病细胞株K_(562)、HL-60增殖抑制作用的实验研究

INHIBITING EFFECT OF SAGO CYCAS LEAVES ON PROLIFERATION OF LEUKEMIA K562 AND HL-60 CELLS

目的:观察铁树叶在白血病细胞株K562及HL-60中的作用。方法:细胞培养液中分别加入铁树叶(实验组1和实验组2)及P.B.S(对照组1和对照组2),观察集落细胞数、死活细胞计数及细胞蛋白质含量。实验组和对照组比较,死活细胞计数和细胞蛋白含量P值均 < 0.05,集落细胞计数实验组抑制率50%。结果:实验组和对照组比较,死活细胞计数和细胞蛋白含量P值均<0.05,集落细胞计数实验组抑制率>50%。结论:铁树叶提取液对白血病细胞株K562及HL-60细胞的增殖有明显抑制作用。

VE:To evaluate effect of sago cycas leaves inhibiting proliferation of leukemia K562 and HL-60 cells. METHOD: The extract of sago cycas leaves was added to K562 and HL-60 cell culture fluid respectively, Cell clusters, live-cells,dead cells were calculated and cell protein was detected. The results were compared with P. B. S. control group. RESULTS: Compared to control group, number of dead and live cells and contents of cell protein were significantly lower in experimental group (P < 0.05). 50% cells in experimental group were inhibited.CONCLUSION: Extract sago cycas leaves has remarkable inhibiting ef-fect on proliferation of leukemia K562 and HL-60 cells.