舌鳞状细胞癌患者血清VEGF水平的定量分析(英文)

A QUANTITATIVE ANALYSIS OF SERUM VASCULAR ENDOTHELIALGROWTH FACTOR LEVELS IN PATIENTS WITH SQUAMOUSCELL CARCINOMA OF THE TONGUE

目的 血管生成是肿瘤发生、发展和转移过程中的关键步骤。最近,许多血管生成因子陆续被分离出来。血管内皮生长因子（VEGF）是其中的一种,对血管内皮细胞的有丝分裂具有选择性作用。本研究目的是检测舌癌患者血清中VEGF含量,并探讨其与舌癌的临床病理特征及预后的关系。方法 研究对象包括10名正常献血员和31名舌癌患者。采用双抗体夹心法 ELISA定量检测血清中 VEGF浓度。结果处理采用独立样本t检验（independent-samples t test）。结果 正常对照组血清中VEGF的平均浓度为148.80±64.17pgml-1（范围,59～231pg.ml-1）,舌癌患者血清中VEGF的平均浓度为567.97±338.17pgml-1（范围,136～1892pg·ml-1）,两者之间的差异具有显著性（P<0.001）。舌癌患者血清中VEGF水平的高低与转移和临床分期密切相关（P<0.05）,而与性别和肿瘤的分化程度无关（P>0.05）。 结论 VEGF水平升高是舌癌发生、发展和转移的重要原因。检测舌癌患者血清中VEGF含量有助于判断有无转移及预后。

ve Angiogenesis is a crucial step in tumor growth, progression, and metastasis . Recently , many angiogenic factors have been identified. Vascular endothelial growth factor (VEGF) is thought to be one such angiogenic factor and is also thought to be a selective mitogen for vascular endothelial cells. The present study was designed to determine the circulating levels of VEGF in human squamous cell carcinoma of the tongue (SCCT) and to investigate its correlation with the clinicopathologic features and prognosis. Methods The study consisted of 10 healthy volunteers and 31 patients with squamous cell carcinoma of the tongue. A quantitative sandwich enzyme immunoassay technique was used to measure the concentrations of VEGF in the sera. The data obtained from the experiment were analyzed with independent-samples t test. Results The mean concentrations of serum VEGF were 148. 80 ± 64. 17pg. ml-1 (range, 59 - 231 pg.ml-1) in normal controls and 567 .97 ± 338 .17pg. ml-1 (range, 136 - 1892pg.ml-1) in SCCT group . Statistical analysis showed that the mean VEGF level in sera of SCCT patients was significantly higher than that in healthy controls ( P < 0.001). The serum VEGF levels of SCCT patients correlated with condition of nodal metastasis (P <0.05) and clinical stage ( P < 0.05) but not with sex ( P > 0.05) and degree of differentiation (P > 0.05) . Conclusion Our study showed that SCCT was associated with significantly elevated serum VEGF concentration. higher level of serum VEGF also correlated with lymph node metastasis and clinical stage of SCCT.