反义bcl-2寡脱氧核苷酸对BcaCD885细胞凋亡的影响

APOPTOSIS INDUCED BY ANTISENSE BCL-2 OLIGODEOXYNUCLEOTIDES IN BcaCD885 CELLS

目的 探讨反义bel-2寡脱氧核苷酸（oligodeoxynucleotide,ODN）对BcaCD885细胞凋亡的影响。方法 以脂质体 Lepofection为载体,一过性转染20μmol/L反义及正义bel-2 ODN于BcaCD885细胞后,通过细胞形态观察及流式细胞术（FCM）分析,从形态学及细胞学水平对凋亡细胞进行检测。结果 20μmol/L反义bel-2 ODN转染组,细胞出现了凋亡形态学改变,FCM检测凋亡率与对照组间存在显著差异（P<0.05）。而 20μmol/L正义bell-2ODN转染组与对照组相似,在形态学上未见明显的凋亡细胞出现,FCM检测两者凋亡率不存在显著差异（P<0.05）。结论 反义 bel-2 ODN能促进 BcaCD885细胞凋亡。

ve To study apoptosis induced by antisense oligodoexynucleotides which complements to translation-initiation site of human bcl-2 mRNA in BcaCD885 cells. Methods First, we transfected 20μmol/L sense bcl-2 oligodeoxynucleotides (5'-CAG CGT GCG CCA TCC TTC CC-3') (sense ODN group) or antisense bcl-2 oligodeoxynucle-otides (5'-GGG AAG GAT GGC GCA CGC TG-3') (antisense ODN group) into BcaCD885 cells through Lepofectin vectin in 37℃ and 5% CO2 conditions for 12 hours. And we used tri-distilled water instead of oligodeoxynucleotides in control group. Then we continued to culture the cells in the same conditions for 24 hours, 36 hours or 48 hours. We studied the characteristics of apoptotic cells with morphological observation (Giemsa coloration) and the cellular apoptotic rate with flow cytometry analysis at the 24h, 36h or 48h. Results In the antisense ODN group, there appeared apoptotic cells and the apoptotic rate was 37.650%, 43.675%, 43.175% respectively at 24h, 36h or 48h after transfection and significantly-raised compared with control group (P <0.05) . But there was no difference between the sense ODN group and the control group (P >0.05). Conclusion Antisense bcl-2 oligodeoxynucleotids can promote apoptosis in BcaCD885 cells.