苯基荧光酮荧光法测定Sn^(2+)H_2O_2体系产生的·OH

Phenyfluorene Fluoremetry Assay of Hydroxyl Radical Produced by Sn^(2+)H_2O_2

提出了一种快速、简便地测定Sn(Ⅱ ) -H2 O2 体系产生羟自由基 (·OH)的新方法。用荧光法建立了测定羟自由基的产生量 ,采用苯基荧光酮 phenylfluorone作为Sn(Ⅱ ) -H2 O2 反应体系的荧光试剂。通过测定苯基荧光酮反应前后的荧光强度变化 ,间接地测定羟自由基产生量。找到了体系最佳实验条件。利用电子自旋共振法ERS ,测定苯基荧光酮加入前后的波谱变化 ,从而进一步证明了该方法的可靠性。同时 ,测定了几种保健药物对羟自由基 (·OH)的清除能力。方法简便 ,测定快速 ,稳定性好 ,灵敏度高 ,无需昂贵的仪器 ,可作为一种筛选抗氧化剂的简便方法 ,亦可作为食品保鲜 ,化妆品性能检验的一种方法。

It was found that the yield of Hydroxyl radicals produced in Sn 2+ -H 2O 2 system was 100 times more than that produced in Fenton reaction.A fluorimetry method for the determination of hydroxyl radicals was developed based Phenyfluorene Sn 2+ H 2O 2 system.The excitation and emission wavelength were chosen at 500nm and 510nm.With measurement of fluorescence intensity, the content of hydroxyl radicals in the solution was successfully determined.The results of clearing expericent and ESR showed that the method was effective and reliable.This sensitive method can be applied to sieve the hydroxyl radical scavenger.