碱性成纤维细胞生长因子对兔下颌骨骨折愈合中细胞增殖的影响

Effect of recombinant human basic fibroblast growth factor on cell proliferation during mandible fracture healing in rabbit

目的 :研究重组人碱性成纤维细胞生长因子 ( recombinant human basic fibroblastgrowth factor,rhb FGF)对兔下颌骨骨折愈合中细胞增殖的影响。方法 :在兔下颌骨骨折动物模型中 ,以牛胶原蛋白 I型为载体 ,外源性应用 rhb FGF于骨折局部。分别于术后 1、2、4、8、12周时取材 ,采用 ABC方法进行增殖细胞核抗原 ( proliferating cell nuclearantigen,PCNA)免疫组化染色。结果 :术后 1、2周时 rhb FGF治疗组骨痂内 PCNA阳性细胞比率明显高于对照组 ( P<0 .0 1)。结论 :rhb FGF在兔下颌骨骨折愈合早期 ,对细胞的增殖起十分重要的促进作用。

objective: To investigate the effect of recombinant human basic fibroblast growth factor (rhbFGF) on cell proliferation during mandible fracture healing. Methods 8 μg of rhbFGF in 0.3 ml of H 2O was mixed with 1.6 ml of bovine type 1 collagen and then the mixture was vacuumed to be a membrane. The membrane was implanted around the mandibular fracture following the fracture was made in 25 rabbits. 5 animals were sacrificed 1,2,4,8 and 12 weeks respectively after operation. Proliferating cell nuclear antigen (PCNA) in callus was examined with immunohistochemical staining. Type 1 collagen membrane was used as the control in another 25 rabbits. Results:1 week after operation, PCNA positive cell(%) in callus in rhbFGF treated and control group were 65.2±6.1 and 32.0±5.5 respectively ( P <0.01),2 weeks after operation,those were 42.4±2.9 and 31.1±3.5 respectively ( P <0.01). 8 and 12 weeks after operation, few PCNA positive cell was observed in both groups. Conclusion:rhbFGF stimulates cell proliferation during mandible fracture healing in rabbit.