骨-软骨复合组织构建的研究

Tissue engineering of bone and cartilage composite

目的 :研究构建骨 -软骨复合组织的可行性。方法 :分别用培养扩增和酶消化法获得成骨细胞和软骨细胞 ,共同接种于珊瑚支架材料上的不同部位 ,然后植入裸鼠体内 ,术后 2个月取材 ,通过大体标本和组织学检查 ,观察骨 -软骨复合组织的构建情况。结果 :2个月后再造出的组织 ,部分为白色 ,表面光滑 ,为软骨组织 ,部分为暗红色 ,质地坚硬 ,为骨组织 ,两部分之间有明显的界限 ;组织学检查结果进一步证实形成了骨 -软骨复合组织。结论 :成骨细胞和软骨细胞共同接种于珊瑚支架材料上 ,可以成功的再造出骨 -软骨复合组织。

砄bjective: To fabricate tissue engineered bone cartilage composite. Method: Rabbit marrow stem cells (MSCs) were in vitro cultured, expanded and induced to differeciate to osteoblasts. Chondrocytes were obtained by collagenase type Ⅱ digestion of rabbit ear cartilage. Osteoblasts and chondrocytes were co seeded into different part of natural coral scaffold, and then implanted subcutaneously into the back of nude mice. Two months after implantation,the specimens were harvested and bone cartilage composites formation was observed by gross inspection and histologic observation. Results: The newly formed tissue was composed of two parts. One part was glisteringly white and another part was dark red. There was an obvious boundary between the two parts. Microscopic observation revealed successful restoration of bone cartilage composite. Conclusion:Bone cartilage composite can be prepared by co deeding of osteoblasts and chondrocytes into natural coral scaffold.