聚乙醇酸负载软骨细胞构建气管软骨环的研究

Fabrication of trachea cartilage ring by tissue engineering

目的 :应用组织工程原理 ,研究软骨细胞接种于聚乙醇酸支架中进行气管软骨环的构建的可行性。方法 :按气管软骨环的形状制备模具 ,将聚乙醇酸无纺网置于模具中 ,用聚乳酸塑形 ;用 型胶原酶消化法收集兔软骨细胞 ,将浓度的 5× 10 7/ ml接种于聚乙醇酸支架中 ,体外继续培养 1周后 ,植入裸鼠背部皮下组织中 ,2个月后取材 ,通过大体标本观察、组织学检查观察气管软骨环的构建情况。结果 :植入后 2个月 ,在裸鼠背部隐约可见形成的环状结构 ,质地较坚韧 ;取材后见形成的组织与气管软骨环基本一致 ,具有良好的弹性和支撑能力 ;组织学检查见新形成的组织由软骨构成。结论 :聚乙醇酸负载软骨细胞 。

砄bjective: To study the feasibility of fabrication of trachea cartilage ring by tissue engineering.Methods : PGA non woven mesh was put into the mold of trachea cartilage ring and enforced with polylactic acid. Rabbit chondrocytes were harvested by collagenase type Ⅱdigestion of ear cartilage and seeded into PGA scaffold in the density of 5×10 7/ml.The cell polymer complexes were incubated in vitro for 1 week and then implanted subcutaniously into the back of nude mice. The formation of trachea cartilage ring was observed by gross inspection and histological examination 2 months after implantation. Results: New cartilage tissue in the shape of trachea ring was found 2 months after implantation. The specimens showed the appearance of glisteringly white with good flexibility. Histological examination demonstrated that newly restored tissue was constituted of cartilage. Conclusion: It may be an efficient method to fabricate trachea cartilage ring by seeding chondrocytes in PGA scaffold.