钙处理对香菇采后氧化酶活性的影响

Effect of postharvest calcium treatments on the activities of catalase,peroxidase, polyphenol oxidase and ascorbic acid oxidase in Lentinula edodes

采用不同浓度的 Ca Cl2 溶液真空浸渗香菇采后子实体 ,以蒸馏水 (CK1 )和 0 .0 0 5 mol· L- 1的钙抑制剂 EG-TA处理 (CK2 )为对照 ,探讨 Ca2 + 处理对香菇采后过氧化氢酶、过氧化物酶、多酚氧化酶、抗坏血酸氧化酶的活性变化的影响。结果表明 ,1与 CK1 比较 ,0 .0 0 5 m ol· L- 1、 0 .0 1m ol· L- 1、 0 .0 3m ol· L- 1、 0 .0 6 mol· L- 1和0 .0 9mol· L- 1 的 Ca Cl2 处理能极显著提高香菇采后过氧化氢酶活性 ,极显著抑制香菇采后过氧化物酶、多酚氧化酶和抗坏血酸氧化酶的活性 ,其中尤以 0 .0 1mol· L- 1、 0 .0 3m ol· L- 1和 0 .0 6 mol· L- 1的 Ca Cl2 处理效果较好 ;0 .12 mol· L- 1 和 0 .15 mol· L- 1 的 Ca Cl2 处理对香菇采后 4种氧化酶活性变化的影响与 CK1 差异不显著。 2与CK2 比较 ,0 .0 0 5～ 0 .15 m ol· L- 1 Ca Cl2 处理均能极显著提高香菇采后过氧化氢酶活性 ,极显著抑制香菇采后过氧化物酶、多酚氧化酶和抗坏血酸氧化酶的活性。 3与 CK1 比较 ,0 .0 0 5 mol· L- 1的 EGTA处理能显著抑制香菇采后过氧化氢酶活性 ,刺激过氧化物酶、多酚氧化酶。

Fruitbodies of Lentinula edodes were vacuum infiltrated with calcium chloride solutions in order to determine the effect of different calcium concentrations on the activities of four oxidases in Lentinula edodes; The results showed that,the activity of catalase was significantly increased,while the activities of peroxidase,polyphenol oxidase and ascorbic acid oxidase were significantly decreased in CaCl 2 treatment at concentrations of 0 005 mol·L -1 ,0 01 mol·L -1 ,0 03 mol·L -1 ,0 06 mol·L -1 and 0 09 mol·L -1 ,the treatment effectivity of 0 01 mol·L -1 ,0 03 mol·L -1 and 0 06 mol·L -1 CaCl 2 were better The activities of these oxidases in Lentinula edodes treated with calcium chloride were not evidently effect at high concentrations of 0 12 mol·L -1 and 0 15 mol·L -1 The activity of catalase was remarkably restrain,and the activities of peroxidase,polyphenol oxidase and ascorbic acid oxidase were markedly inhance in Lentinula edodes treated with 0 005 mol·L -1 EGTA