中国野桑蚕和家蚕的AFLP分析

AFLP Analysis of Chinese Mulberry Wild Silkworm ( Bombyx mandarina )and Domestic Silkworm( Bombyx mori )

利用AFLP技术和统计学分析原理 ,对我国具有代表性的 10个不同地区野桑蚕和 33个家蚕品种以及 5个作为外群对照的柞蚕品种进行了分子系统学研究。研究结果表明 ,不同地区野桑蚕之间的遗传距离 (0 0 0 0～0 419)与家蚕品种之间的遗传距离 (0 0 0 0～ 0 40 6 )相似 ,而家蚕与野桑蚕之间的遗传距离为 (0 35 5～ 0 5 32 ) ,明显地小于家蚕与柞蚕 (0 76 1～ 0 86 5 )和野桑蚕与柞蚕 (0 776～ 0 839)之间的遗传距离 ,进一步证明了家蚕起源于中国野桑蚕。聚类分析发现 ,中国一化种与二化种聚类在一起 ,中国二化种与热带种聚类在一起 ,热带种不与一化种聚在一个类群 。

Based on 5 strains of tussah( Antheraea pernyi )as the outgroups,the molecular systematic studies of Chinese mulberry wild silkworm from 10 regions in China and 33 strains of domestic silkworm were performed by AFLP technique and statistical method in the paper.The results showed that the genetic distance of mulberry wild silkworm between different regions is similar to that of domestic silkworm between strains,while the genetic distance between mulberry wild silkworm and domestic silkworm is much shorter than that both between domestic silkworm and tussah and between mulberry wild silkworm and tussah.The results confirmed that domestic silkworm originated from Chinese mulberry wild silkworm.Meanwhile,clustering analysis found that Chinese monovoltine strains cluster together with Chinese bivoltine strains,Chinese bivoltine strains cluster together with tropical strains,and Chinese monovoltine strains and tropical strains cant cluster a cluster.It is proved that Chinese bivoltine strain is between monovoltine strain and tropical strain in evolution.