化呆醒神汤对大鼠血管性痴呆模型病理变化及胆碱能纤维的影响

Effects of Huadaixingshen Decoction on the Pathological Changes and Cholinergic Fibers in the Rats with Vascular Dementia

本实验采用反复缺血再灌方法复制出血管性痴呆 (VD)动物模型 ,以具有调理脾胃、消痰化瘀功效的化呆醒神汤进行治疗。光、电镜观察发现VD大鼠海马CA1区大量神经元出现变性、坏死及脱失 ,线粒体肿胀、空泡样变性、突触变性 ;组织染色及定量分析发现VD大鼠海马CA1区、额叶皮质内乙酰胆碱酯酶 (AchE)阳性纤维数量显著减少。化呆醒神汤可以明显减轻VD大鼠海马CA1区神经元损伤 ,增加海马CA1区、额叶皮质内AchE阳性纤维数量。

Rat vascular dementia model was established by the repeated cerebral ischemia reperfusion method, and the model rats were treated with Huadaixingshen Decoction (HD) with the administration of hydergine as contrast. The results of the observation with the optical microscope and the electron microscope showed that in the rats of the model group, a large number of neurons in the hippocampal CA1 area exhibited degeneration, necrosis and deletion, mitochondrial tumefaction, vacuolar degeneration and synaptic degeneration; and the tissue staining and quantitative analysis found that the number of AchE positive fibers in the hippocampal CA1 area and the frontal cortex obviously decreased. It was shown that HD could obviously reduce the neuronal injury in the hippocampal CA1 area, and increase the number of AchE positive fibers in the hippocampal CA1 area and the frontal cortex, and its effects were better than those of hydergine.