滋肾活血解毒方对实验性慢性肾衰大鼠IL-8、TNFα及SOD的影响

Effects of Zishenhuoxuejiedu Recipe on the Levels of Il-8, TNFα and SOD in Rats with Experimental Chronic Renal Failure

采用 5/ 6肾切除方法复制大鼠CRF动物模型 ,并随机分成滋肾活血解毒方、洛汀新、保肾康、病理对照及正常对照组 ,用放射免疫分析法检测血中IL 8、TNFα及SOD水平。结果示各CRF模型组大鼠血中IL 8、TNFα水平高于正常对照组 ,血中SOD水平低于正常对照组 ;滋肾活血解毒方、洛汀新组大鼠血中Cr、BUN水平低于保肾康及病理对照组 ;滋肾活血解毒方组大鼠血中IL 8、TNFα水平明显低于洛汀新、保肾康及病理对照组 ,而血中SOD水平明显高于 3组对照。结果表明滋肾活血解毒方有明显改善肾功能的作用 ,其机理与降低或阻断IL 8、TNFα分泌 ,抗细胞组织过氧化 ,减轻肾小球硬化有关。

The rat model of chronic renal failure was created by the method of cutting off 5/6 of the kidney, and the model rats were randomly divided into 5 groups, namely Zishenhuoxuejiedu Recipe (ZR), lotensin, Baoshenkang, pathological control and normal control, in which the serum levels of Il 8, TNFα and SOD were respectively determined by radioimmunoassay. The results showed that the serum Il 8 and TNFα levels in the former 4 groups were higher than those in the normal control group, and the serum SOD level in the former 4 groups was lower than that in the normal control group; the serum Cr and BUN levels in the ZR and lotensin groups were lower than those in the Baoshenkang and pathological control groups; the serum Il 8 and TNFα levels in the ZR group were obviously lower than those in the lotensin, Baoshenkang and pathological groups, while the SOD level in the ZR group was obviously higher than that in these 3 groups. The results suggest that ZR has marked effects of improving renal function.