苯并(a)芘代谢物反式二羟环氧苯并芘诱发人支气管上皮细胞恶性转化

Malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene metabolite dihydroxyepoxy benzo pyrene

以不同浓度的苯并 (a)芘代谢物反式二羟环氧苯并芘 (BPDE)多次处理人支气管上皮细胞 16HBE ,并观察转化细胞的恶性特征。发现BPDE可诱导 16HBE细胞恶性转化 ,形成转化灶。转化灶细胞失去接触抑制 ,排列紊乱 ,无方向性 ,交叉重叠生长。转化的细胞可在软琼脂上生长 ,各浓度处理组细胞集落形成率均显著高于对照组 ,有良好的剂量—反应关系。经BPDE处理的细胞在裸鼠体内成瘤 ,病理学诊断为鳞状细胞癌。本实验以反式BPDE成功地诱发了人支气管上皮细胞恶性转化 ,为后期进一步研究其致癌的分子机制、寻找致癌相关基因提供了理想的生物学材料。

The malignant transformation of human bronchial epithelial cell 16HBE induced by benzo (a) pyrene metabolite——dihydroxyepoxy benzo pyrene (BPDE) was studied. 16HBE cells were treated several times with BPDE at different concentrations in vitro. The identification on the malignancy of transformed 16HBE cells was investigated. The transformed cells in foci were proliferated rapidly, exhibited in extensive random orientation and crossed over. The transformed cells could grow on soft agar. The colony forming frequency of each BPDE group on soft agar was significantly higher than that of control group and showed dose reaction relations with the concentrations of BPDE. The transformed cells could grow into tumors in nude mice. The tumor was squamose carcinoma confirmed by histopathological examination. The culture system in this study provided a potential tool for studying the molecular mechanism of carcinogenesis of human bronchial epithelial cells induced by BPDE.