Al(NO_3)_3对大鼠胚胎的发育毒性研究

Developmental toxicity of Al(NO_3)_3 on rat embryos

应用体外全胚胎培养方法 ,以Al(NO3) 3为受试物 ,培养基中Al3+ 浓度为 0 6～ 9 0 μg ml,直接染毒旋转培养的大鼠胚胎 48h ,观察Al(NO3) 3对大鼠胚胎生长发育和形态分化的影响 ,以探讨铝的胚胎发育毒性及其机理。结果发现 ,随着铝剂量增加 ,胚胎生长发育和器官分化的各项指标均呈现下降趋势 ,有一定的剂量 效应关系。其中 ,胚胎卵黄囊、神经管及心脏分化发育等对铝的作用较为敏感 ,相关指标在Al3+ 浓度为1 2 μg ml时比对照组显著降低 (P <0 0 5 )。≥ 3 0 μg ml时 ,除下颌外各项指标均明显降低 (P <0 0 5或P <0 0 1) ,同时胚胎畸形发生率明显升高 ,畸形以神经管闭合不全、体屈异常多见。表明Al(NO3) 3可导致胚胎发育迟缓和神经系统等器官发育畸形。

The developmental toxicity and its mechanism of Al(NO 3) 3 on embryos in SD rats were studied. On the 9 5th day of gestation, the embryos were incubated in a whole embryo culture system with Al(NO 3) 3 at concentrations of Al 3+ from 0 6 to 9 0 μg/ml in culture media for 48hrs. Viable embryos were evaluated by Brown's morphological scoring system, and the diameter of yolk sac, crown rump, head length and the dry weight of embryos were measured. There was a dose dependent relations of decreasing embryo development with increasing concentrations of Al 3+ . Yolk sac diameter, head length ,dry weight and heart, forelimb as well as neural tube scores decreased significantly at 1 2μg/ml (P<0 05). When embryos were exposed to Al 3+ at ≥3 0μg/ml, the embryonic development and morphological differentiation were obviously inhibited( P <0 05); meanwhile, the incidence of dysmorphogenesis significantly increased, including neural tube defects and dorsiflexion teratogenesis. The results suggested that aluminum might be a developmental toxicant and dysmorphogenesis agent.