硒对氟致小鼠学习记忆损伤的改善作用

Effects of selenium on the damage of learning-memory ability of mice induced by fluoride

选用Y 迷宫观察硒对氟致小鼠学习记忆能力的影响 ,并用透射电镜和计算机图像分析仪观测分析其学习记忆相关脑区 海马CA3区GrayI型突触的结构变化。结果显示 :较高浓度氟可使小鼠学习能力损伤 ,并能引起海马CA3区突触后致密物质厚度显著变小及其突触间隙宽度显著加大。给氟同时给予适当浓度的硒可拮抗氟的这种作用 ;而高浓度硒与氟产生协同毒性作用。本研究在行为和形态学方面为硒拮抗氟的神经毒性提供证据。

Sodium fluoride added with or without sodium selenite in deionized water was administered to male mice for 8 weeks. The influences of fluoride on learning memory behavior were tested on Y maze,and the ultrastructure of Gray I synaptic interface in the CA 3 area hippocampus was quantitatively analyzed by electron microscopy and computer image processing appliance. The main results showed that the learning capability of mice drinking higher concentration of fluoride presented remarkable deterioration. The thickness of post synaptic density (PSD) was decreased. The width of the synaptic cleft was remarkably increased. It was found that combined administration of fluoride and proper concentration of selenium could decrease the toxic effect of fluoride. There were synergetic toxicities if the concentration of selenium was too high. The results suggested that selenium might antagonize the neurotoxicity of fluoride on behavior and morphology.