茶多酚、茶色素对大鼠肝癌癌前病变抑制作用及机理研究

Studies on the inhibitory effects of tea polyphenols and tea pigments on liver precancerous lesion in rats

用腹腔注射二乙基亚硝胺及四氯化碳和部分肝切除建立的改良Solt Farber大鼠肝癌癌前病变模型以观察茶多酚和茶色素对其抑制作用 ,并对其可能机制进行探讨。结果表明 ,与阳性对照组相比 ,饮0 1%茶多酚和茶色素动物的谷胱甘肽硫转移酶P型 (GST P)阳性灶的面积和数目显著减少。Westernblot分析表明茶多酚和茶色素诱导了p2 1WAF1 表达 ,抑制了Bcl 2蛋白的表达 ,诱导了Bax蛋白的表达。本研究表明茶多酚和茶色素对大鼠肝癌癌前病变具有明显的抑制作用 ,而抑制细胞增殖、诱导细胞凋亡可能是其重要机制。

The chemopreventive effects of tea polyphenols and tea pigments on liver precancerous lesion in rats were investigated . The results showed that the density and area of GST P in the tea treated groups were significantly reduced as compared with the positive control group. Furthermore, tea polyphenols and tea pigments induced the expression of p21 WAF1 protein, inhibited the expression of Bcl 2 protein and induced the expression of Bax protein. It is concluded that GST P was significantly inhibited by tea polyphenols and tea pigments and inhibition of cell proliferation and induction of apoptosis may be the two important mechanisms.