支原体感染与人胃肠肿瘤的关系

Mycoplasma infection in human gastriointestinal carcinoma tissues

目的 检测胃肠肿瘤组织支原体的感染 ,探讨支原体感染与胃肠肿瘤发生的相关性。方法 抗肿瘤单克隆抗体 (McAb)PD4的抗原P40通过测序 ,Hoechst2 2 35 8荧光染色支原体及支原体DNA扩增等方法被证明为猪鼻支原体。利用PD4单抗通过免疫组织化学 ,检测石蜡包埋的人胃肠道的肿瘤组织的支原体感染 ,并用支原体特异性引物对石蜡包埋组织通过PCR扩增支原体DNA片段 ,进行进一步验证。以胃炎、胃溃疡、胃肠上皮化生及腺瘤性息肉标本为对照。结果 在检测的标本中 ,5 6 % (5 0 /90 )的胃癌组织有支原体感染 ,感染随分化程度的降低而降低 ,在对照的胃炎组的感染率为 2 8% (13/4 7) ,肠上皮化生组为 37% (18/4 9) ,胃溃疡组为 30 % (14/4 6 ) ,与胃癌组比较差异均有显著意义 (P <0 0 5 )。5 5 1% (32 /5 8)的肠癌组织有支原体感染 ,感染随分化程度的降低而降低 ,对照的肠腺瘤性息肉组感染率为 2 0 4% (10 /4 9例 ) ,与肠癌比较差异有显著意义 (P <0 0 0 5 )。结论 胃肠道肿瘤组织中支原体感染较非肿瘤疾病有显著提高 ,提示支原体感染与肿瘤发生可能有一定的相关性。

Objective To explore the association between the carcinoma and mycoplasma infection by immunohistochemistry. Methods Antigen of anti tumor monoclonal antibody PD4 was identified as mycoplasma hyorhinis. Using immunohistochemistry to study archived paraffin embedded gastriointestinal tract carcinomas tissue. The PCR was applied to amplify the mycoplasma DNA for confirming immunohistochemistry results. Results It was showed that mycoplasma was present in 56%(50/90 cases) of gastric carcinoma. The cancer tissues with high differentiation had a higher mycoplasma infection ratio than that of low differentiation cancer tissues. ( P <0 05). In control cases, mycoplasma infection was 28%(18/49) in chronic superficial gastritis, 30%(14/46) in gastric ulcer and 37% (18/49) in intestinal metaplasia of the stomach. Mycoplasma infection was 55.1%(32/58) in colon carcinoma and 20.9%(10/49) in adenomarous polyp ( P <0.005). It seems that colon carcinoma tissues with slight pathological grade had a higher percent of mycoplasma infection than that of cancer tissues with moderate and heavy pathological grade ( P <0.05). Conclusion The high infection of mycoplasmas in carcinoma tissues suggest an association between mycoplasma and cancer. The mechanism involved in oncogenesis by mycoplasmas remains to be elucidated.