组织工程用牛心包材料体外降解规律的初步探索

A Study on the Degradation of Bovine Pericardium in Vitro

目的研究牛心包材料在体外的降解规律 ,以进一步对其控制降解并引导组织再生。方法 1 .选择胶原酶、透明质酸酶、木瓜蛋白酶和胰酶 ,在 37℃ ,3、8、1 5、30d ,对不同交联度的牛心包材料进行处理。主要用失重法、平均孔径法、分光光度法、红外测试及扫描电镜来表征材料的各种降解特性。 2 .不同交联度的心包材料在PBS溶液中 37℃下进行水解 ,采用失重法 ,分光光度法表征心包材料的水解规律。结果 30d内实验表明在没有酶的作用下 ,未交联的心包材料基本稳定 ,很难发生大分子断裂的降解 ,仅为小分子溶解和扩散过程。酶解的效果显著 ,其中以胰酶最为明显 ,并得到了几种酶的降解失重曲线。酶解产物经分析为小分子的氨基酸、短肽、多糖类。

Objective To study the degradation of bovine pericardium in PBS solution without enzyme or with different enzymes. Method Four kinds of bovine pericardium with different degrees of crosslinking were obtained by treatment with different concentrations of GA (glutaraldehyde). Two methods, i.e. enzymolysis and non enzyme hydrolysis, were used to dispose the bovine pericardium. Four types of enzymes, i.e. collagenase, hyaluronidase, trypsin and papain, were used to degrade the bovine pericardium in 3, 8, 15, 30 d, (37℃) and then the remaining materials were hydrolyzed in PBS solution (37℃). Weight lose, spectrophotometric method i.e. IR, UV and SEM were used to characterize the degraded materials. Result The results showed that the materials were not easy to be hydrolyzed while they were remarkably degraded under enzyme catalysis. Several degradation curves were drawn which showed that trypsin was most effective while hyaluronidase was least. Four different enzymes had different surface morphologies through SEM. The degradation products were regarded as amino, peptide and polyose by the analysis. Conclusion The result of the experiment showed that bovine pericardium with different degrees of crosslinking degraded under catalysis by different enzymes.