中耳胆脂瘤上皮细胞增生与凋亡状态的研究

A study on the state of cell proliferation and apoptosis in human middle ear cholesteatoma

目的 :研究中耳胆脂瘤上皮细胞增生和凋亡的状态。方法 :应用免疫组化染色 SABC技术及原位凋亡细胞标记技术 (TU NEL 法 ) ,对 2 0例中耳胆脂瘤上皮组织和 10例外耳道正常上皮组织样本进行研究。结果 :在中耳胆脂瘤上皮中 ,增殖细胞核抗原 (PCNA )阳性细胞大量存在于基底细胞层、棘细胞层及颗粒细胞层 ,而在正常外耳道上皮中 PCNA阳性细胞仅存在于基底层 ;在胆脂瘤上皮中 PCNA阳性细胞率和平均光密度分别为(36 .91± 2 2 .77) %和 0 .2 4 2 7± 0 .0 5 86 ,明显高于正常外耳道上皮中的 PCNA阳性细胞率 (10 .2 5± 2 .6 5 ) %及平均光密度 (0 .1340± 0 .0 36 3) ,其差异有显著性意义 (P<0 .0 5 )。同时 ,在胆脂瘤上皮中 ,凋亡细胞存在于棘细胞层及颗粒细胞层 ,而外耳道正常上皮与之相似 ,但两者的凋亡率分别为 (2 7.5 0± 12 .5 0 ) %和 (9.96± 3.86 ) % ,其差异有显著性意义 (P <0 .0 5 )。结论 :中耳胆脂瘤上皮具有高度增生和凋亡的能力 ,并因上皮细胞的增生、分化。

Objective:To study the state of proliferation and apoptotic cell death in cholesteatoma. Method:20 cholesteatoma samples and 10 normal external ear canal skin samples were studied with immunohistochemical SABC technique and TUNEL technique.Result:Immunohistochemical analysis using a monoclonal antibody to proliferation cell nuclear antigen (PCNA)demonstrated the presence of proliferating cell in the basal cell layer of normal external ear canal skin,whereas in the cholesteatoma tissue samples larger numbers of proliferation cell nuclear antigen positive cells were also observed in the prickle and granular cell layers. The PCNA labeling index and the mean optical density were ( 10.52 ± 2.65 )% and ( 0.134 0 ± 0.036 3 ) in normal external ear canal skin samples and ( 36.91 ± 22.77 )% and ( 0.242 7 ± 0.058 6 ) in cholesteatoma tissue samples.In cholesteatoma tissue samples and normal external ear canal skin samples, apoptotic cell were observed in the prickle and granular cell layers.The apoptotic indexers were ( 27.50 ± 12.50 )% in cholesteatoma tissue samples and ( 9.96 ± 3.86 )% in skin samples. Conclusion:The results demomstrate the rates of apoptotic cell death and cell proliferation in cholesteatoma are higher than those in normal skin. Increased cell proliferation and cell death will contribute to increased keratous debris.