一侧迷路切除对前庭内侧核NMDA受体亚单位R1mRNA表达的影响

The influence of labyrinthectomy on the expression of NMDA R1 mRNA

目的 :研究豚鼠一侧迷路切除对前庭内侧核区 N-甲基 - D-天门冬氨酸 (NMDA)受体亚单位 R1m R-NA表达的变化。方法 :利用原位杂交方法 ,切除豚鼠一侧迷路 ,观察前庭内侧核区 NMDA受体亚单位 R1m RNA的变化 ,及其在前庭代偿中可能的作用。结果 :手术组一侧迷路切除 ,2 0～ 4 0 h,受损侧前庭内侧核表达阳性细胞数较未损侧明显增多 (P <0 .0 1) ;4 0 h～ 6 d处于下降趋势 ,到 6 d与对照组差异已无显著性意义 (P >0 .0 5 )。结论 :前庭内侧核区 NMDA受体亚单位 R1表达的变化趋势与前庭静态症状的恢复变化趋势基本一致 ,表明 NM-DA受体亚单位

Objective:To study the effect of labyrinthectomy on the expression of NMDAR1(N methyl D sapartate receptor subunit R1).Method:The in situ hybridization was used to observe the expression of NMDA R1mRNA post unilateral labyrinthectomy and investigate its effect on vestibular compensation.Result:20 h post unilateral labyrinthectomy, the NMDA R1mRNA positive neurons in the medial vestibular nucleus increased significantly compared with the unlesioned side(P< 0.01 ).40 h～6 d,the positive cells decreased gradually. 6 d post lesion, the positive neuron counts of the lesioned side had no significant difference compared with those of the un lesioned side (P> 0.05 ).Conclusion:The changing trend of the expression of NMDA R1mRNA in the medial vestibular nucleus is related to the recovery of the vestibular static symptoms after unilateral labyrinthectomy.It is suggested that the modulation of the efficacy of the NMDA R1 play an important role in the compensation of the static symptoms.