急性髓系白血病相关逆转录病毒基因表达的研究

Study on the expression of human acute myeloid leukemia retrovirus gene

目的 探索急性髓系白血病 (AML)逆转录病毒病因的可能性。方法 运用已获得的逆转录病毒 6 # 11克隆为探针和引物 ,通过Northernblot和RT PCR技术检测AML患者白血病细胞中相关逆转录病毒基因的表达情况。结果 以 6 # 11为探针 ,Northernblot杂交的阳性条带在 9.4kb和 4.5kb左右 ,19例AML患者白血病细胞中 18例出现了阳性条带 ;RT PCR设计的引物扩增片段长度为 790bp ,14例AML患者白血病细胞中阳性表达 11例。结论 AML患者白血病细胞中相关逆转录病毒基因呈特异性表达。

Objective To explore the potentiality of retroviral etiology in human acute myeloid leukemia(AML). Methods The expression of retrovirus clone 6#11 in leukemic cells from 19 AML patients and peripheral blood mononuclear cells (PBMNCs) from 20 controls was studied by Northern blot and reverse transcriptase polymerase chain reaction (RT PCR). Results Northern blot revealed the positive bands of 9.4 kb and 4.5 kb in 18 of 19 AML patients, and RT PCR a 790 bp product in 11 of 14 AML patients. Conclusion There was a specific expression of acute myeloid leukemia associated retrovirus in AML patients.