脑源性神经营养因子基因修饰细胞移植治疗大鼠脊髓损伤的电生理研究

Intraspinal implantation of genetically modified myoblasts with brain-derived neurotrophic factor gene in treating spinal cord injury in rats: neurophysiological study

目的 采用神经电生理检查方法 ,观察逆转录病毒载体介导脑源性神经营养因子(BDNF)基因修饰成肌细胞对损伤脊髓的治疗作用。 方法 30只SD大鼠在T9水平制成脊髓横断损伤模型并随机分为基因细胞组 (A组 )、成肌细胞组 (B组 )及损伤对照组 (C组 ) ,每组 10只大鼠。术后 3个月 ,采用皮层体感诱发电位 (CSEP)和运动诱发电位 (MEP)等电生理检测技术 ,观察轴突是否有再生及其神经功能恢复程度。 结果 (1)A组中 3只大鼠损伤 3个月后出现CSEP波 ,5只出现MEP波 ,B、C组动物未发现电生理信号恢复 ;(2 )重新出现的CSEP或MEP信号均较损伤前波幅减低 ,潜伏期延长 ;(3)A组脊髓神经功能较B、C组有显著改善。

Objective To observe the value of gene therapy to spinal cord injury in vivo by transferring gene of brain derived neurotrophic factor (BDNF) to rat myoblast with retroviral vector electrophysiologically. Methods Transection of spinal cord at the level of T 9 was produced in 30 adult SD rats that were divided randomly into three groups: BDNF gene modified myoblast implantation (group A), myoblast implantation (group B), and no cell implantation as controls (group C). Three months after operation, whether the axon had regenerated and how the neural function was restored were observed, using the techniques of cerebral somatosensory evoked potential (CSEP) and motor evoked potential (MEP). Results Three months after injury, 3 rats in group A showed CSEP signals and 5 rats MEP signals, but no electrophysiological response was found in group B or group C. The amplitudes of the recovery of CSEP or MEP signals decreased and their latencies increased as compared with those before injury. The neural function of the spinal cord was greater in group A than in B and C. Conclusion Treatment with genetically modified myoblasts producing BDNF can accelerate recovery from traumatic spinal cord injury in adult rats.