利用RAPD技术进行野生大豆种群内分化的研究

RESEARCH OF RAPD IN LOCAL POPULATION DIFFERENTIATION OF WILD SOYBEAN

利用RAPD技术对 2 5°N野生大豆种群 1 6个样本进行分子标记 .从 1 5 0多个RAPD引物中筛选出具有多态性的引物 2 0个 ,共扩增出 1 46条带 ,其中具有多态性带 60条 ,点 40 .8% .平均遗传距离为0 .1 5 3 6;杂合度为 0 .3 2 48,聚类分析结果表明 2 5°N野生大豆 1 6个样本分成 4类 ,种群内存在大量的遗传变异 ,本实验为研究野生大豆种群内及进一步研究种群间的遗传多态性探索了一种可行的方法 。

Random amplified polymorphic DNA(RAPD) analyses were applied to 16 samples of wild soybeans (Glycine soja siec & Zucc) originated from twenty_five degrees of latitude.Twenty primers screened with 150 ones yielded 146 RAPD bands which had 60 polymorphic products and 40.8% of total bands.The average heterozygosity (He) was 0.3248 and the average genetic distance among the 16 samples was 0.1536.Cluster analysis showed there were four small classes divided from the 16 samples and there were some evidence showing genetic variations in the population.It was concluded genetic resources was also put forward.