摘要
利用RAPD技术对 2 5°N野生大豆种群 1 6个样本进行分子标记 .从 1 5 0多个RAPD引物中筛选出具有多态性的引物 2 0个 ,共扩增出 1 46条带 ,其中具有多态性带 60条 ,点 40 .8% .平均遗传距离为0 .1 5 3 6;杂合度为 0 .3 2 48,聚类分析结果表明 2 5°N野生大豆 1 6个样本分成 4类 ,种群内存在大量的遗传变异 ,本实验为研究野生大豆种群内及进一步研究种群间的遗传多态性探索了一种可行的方法 。
Random amplified polymorphic DNA(RAPD) analyses were applied to 16 samples of wild soybeans (Glycine soja siec & Zucc) originated from twenty_five degrees of latitude.Twenty primers screened with 150 ones yielded 146 RAPD bands which had 60 polymorphic products and 40.8% of total bands.The average heterozygosity (He) was 0.3248 and the average genetic distance among the 16 samples was 0.1536.Cluster analysis showed there were four small classes divided from the 16 samples and there were some evidence showing genetic variations in the population.It was concluded genetic resources was also put forward.
出处
《松辽学刊(自然科学版)》
2001年第4期1-4,共4页
Songliao Journal (Natural Science Edition)
基金
国家自然科学基金资助项目 ( 39730 330
39670 44 8)
"973"资助项目 (G19980 10 2 0 0 )
关键词
RAPD
种群分化
野生大豆
分子标记
RAPD
glycine soja
poulation differentiation
molecular markers