肝癌相关性甘氨酰脯氨酸二肽氨基肽酶检测法

DETECTION OF HEPATOMA-ASSOCIATED GLYCYLPROLINE DIPEPTIDYL AMINOPEPTIDASE

目的 :进一步探讨血清肝癌相关性 GPDA同工酶分离检测条件 ,提高其对原发性肝癌的诊断价值。方法 :对分离血清肝癌相关性 GPDA同工酶电泳体系的凝胶浓度及梯度、缓冲液的 p H及离子强度、电泳的环境温度、标本用量以及呈色条件等进行了逐项摸索 ,建立了稳定且分离效果好的 GPDA同工酶检测方法。结果 :采用三层阶段梯度聚丙烯酰胺凝胶体系将血清 GPDA分为肝癌相关性的快带 (GPDA- F)和非相关性的慢带 (GPDA- S) ,肝癌相关性 GPDA的检出率明显提高。结论 :肝癌相关性血清 GPDA检测法稳定可靠、敏感 。

Objective: To explore the detection conditions for serum hepatoma-associated glycylproline dipeptidyl aminopeptidase (GPDA) isoenzyme to enhance its diagnostic value for primary hepatic carcinoma. Methods: Gel concentration and gradients, pH and ion strength of buffers, environmental temperature, applied serum volume and staining conditions for detection of GPDA isoenzymes were studied. Results: Serum GPDA was separated into hepatoma-associated fast band (GPDA-F) and non-associated slow band (GPDA-S). The detection rate of hepatoma-associated GPDA is much higher by the modified method than by the former method. Conclusions: The modified method for detection of serum hepatoma-associated GPDA is stable and sensitive, and it is suitable for the clinical use.