摘要
目的 探讨 Janu2亚型酪氨酸激酶在 Ca2 + 池操纵性 Ca2 + 内流中的作用。 方法 记录大鼠主动脉环收缩反应。 结果 (1)不同剂量 Janu2亚型酪氨酸激酶抑制剂 tyrphostin AG4 90 (AG4 90 ,1~ 30μmol/ L )以浓度依赖性抑制环匹阿尼酸 (CPA)触发的大鼠主动脉血管环收缩持续相。 AG4 90的最大作用浓度为 10μmol/ L ,抑制率为 4 9%± 12 %。 (2 ) 10μm ol/ L AG4 90可显著抑制高 KCl触发的大鼠主动脉血管环收缩反应。 (3) 10μm ol/ LAG4 90和 1μm ol/ L硝苯地平对 CPA触发收缩反应的抑制作用存在部分重叠。 结论 CPA触发平滑肌的收缩过程中 ,蛋白质 Janu2亚型酪氨酸激酶参与了开启电压依赖性 Ca2 + 通道和受体操纵性 Ca2 +
Objective\ To study the relationship between store\|operated Ca\+\{2+\} influx and tyrosine kinase inhibitor of Janu2 type in rat vascular smooth muscle.\ Methods\ The tension of rat aortic ring was measured.\ Results\ (1)Tryphostin AG490(AG490,1~30 μmol/L), a tyrosine kinase inhibitor of Janu2 type, produced a concentration\|dependent inhibitory effect on 10 μmol/L cyclopiazonic acid\|induced response.\ The maximal inhibitory effect(49%±12%) of AG490 reached at a concentration of 10 \{μmol/L\}.\ (2)10 μmol/L AG490 obviously inhibited 100 mmol/L KCl\|induced contractile response.\ In this case, addition of 1 μmol/L nifedipine depressed the contractile response completely.\ (3)There was an overlap between the effects of 10 μmol/L AG490 and 1 μmol/L nifedipine on cyclopiazonic acid\|induced contractile response.\ Conclusion\ Tyrosine kinase of Janu2 type participates the signal transduction in the process of voltage\|dependent Ca\+\{2+\} channels and store\|operated Ca\+\{2+\} channels opening which mediates store\|operated Ca\+\{2+\} influx.
出处
《福建医科大学学报》
2001年第4期307-310,共4页
Journal of Fujian Medical University
基金
国家自然科学基金资助项目 (3 9770 85 8)
福建省卫生厅科研基金资助项目 (970 49)