Janu2亚型酪氨酸激酶在环匹阿尼酸触发的大鼠主动脉血管环收缩效应中的作用

Effects of Tyrosine Kinase of Janu 2 Type on Cyclopiazonic Acid-induced Contractile Response in Rat Aortic Smooth Muscle

目的 探讨 Janu2亚型酪氨酸激酶在 Ca2 + 池操纵性 Ca2 + 内流中的作用。 方法 记录大鼠主动脉环收缩反应。 结果 (1)不同剂量 Janu2亚型酪氨酸激酶抑制剂 tyrphostin AG4 90 (AG4 90 ,1～ 30μmol/ L )以浓度依赖性抑制环匹阿尼酸 (CPA)触发的大鼠主动脉血管环收缩持续相。 AG4 90的最大作用浓度为 10μmol/ L ,抑制率为 4 9%± 12 %。 (2 ) 10μm ol/ L AG4 90可显著抑制高 KCl触发的大鼠主动脉血管环收缩反应。 (3) 10μm ol/ LAG4 90和 1μm ol/ L硝苯地平对 CPA触发收缩反应的抑制作用存在部分重叠。 结论 CPA触发平滑肌的收缩过程中 ,蛋白质 Janu2亚型酪氨酸激酶参与了开启电压依赖性 Ca2 + 通道和受体操纵性 Ca2 +

Objective\ To study the relationship between store\|operated Ca\+\{2+\} influx and tyrosine kinase inhibitor of Janu2 type in rat vascular smooth muscle.\ Methods\ The tension of rat aortic ring was measured.\ Results\ (1)Tryphostin AG490(AG490,1~30 μmol/L), a tyrosine kinase inhibitor of Janu2 type, produced a concentration\|dependent inhibitory effect on 10 μmol/L cyclopiazonic acid\|induced response.\ The maximal inhibitory effect(49%±12%) of AG490 reached at a concentration of 10 \{μmol/L\}.\ (2)10 μmol/L AG490 obviously inhibited 100 mmol/L KCl\|induced contractile response.\ In this case, addition of 1 μmol/L nifedipine depressed the contractile response completely.\ (3)There was an overlap between the effects of 10 μmol/L AG490 and 1 μmol/L nifedipine on cyclopiazonic acid\|induced contractile response.\ Conclusion\ Tyrosine kinase of Janu2 type participates the signal transduction in the process of voltage\|dependent Ca\+\{2+\} channels and store\|operated Ca\+\{2+\} channels opening which mediates store\|operated Ca\+\{2+\} influx.