人角蛋白K6a cDNA的克隆及其在E.coli中的表达

Cloning and expression of the cDNA of human keratin 6 a in E.coli

目的 克隆并表达人角蛋白 K6 a c DNA.方法 从人角质形成细胞中提取 RNA,以 RT- PCR法扩增人角蛋白K6 a(1 .7kb) c DNA.测序正确 ,将该片段克隆入表达载体p RSETB中 ,用 IPTG诱导表达 .结果 获得了角蛋白 K6 a(1 .7kb) c DNA片段 ,并在大肠杆菌中获得高效表达 .结论 获得了人角蛋白 K6 a(1 .7kb) c DNA片段及其原核表达产物 ,对研究人角蛋白 K6

AIM To clone and express the 1.7 kb cDNA fragment of human keratin 6 a. METHODS The 1.7 kb cDNA fragment of human keratin 6 a was amplified from keratinocyte total RNA by RT PCR, then it was cloned into expression vector pRSETB and induced with IPTG. RESULTS The 1.7 kb cDNA fragment of human keratin 6 a was cloned. The sequencing indicated that the sequence was identical to that of reports. Then the 1.7 kb cDNA was cloned into expression vector pRSETB. The keratin 6 a fragment was highly expressed in E.coli after induced with IPTG. CONCLUSION The 1.7 kb cDNA fragment of human keratin 6 a is obtained, and it is highly expressed in E.coli , it might be important for study on the function of keratin 6 a.