HPLC法测定胰岛素脂质体中胰岛素的含量

Determination of insulin entrapped in liposome by HPLC

目的 :胰岛素脂质体中胰岛素含量的测定。方法 :采用高效液相色谱法 ,色谱柱为 Hypersil ODS2 ,流动相为 0 .1mol L- 1磷酸二氢钠 - 0 .0 5 m ol L- 1硫酸钠 -乙睛 =36∶ 36∶ 2 8,流速为 1m l m in- 1 ,检测波长为 2 14 nm。结果 :高、中、低的回收率分别为99.5 5 % ,98.2 3% ,10 0 .9% ,RSD分别为 1.1% ,1.7%和 0 .6 1%。在 5～ 10 0μg m l- 1 测定范围内 ,线性良好 ,r=0 .9999。结论 :方法可靠 。

OBJECTIVE:To establish a new method for the determination of insulin entrapped in liposome. METHOD:The chromatographic column was Hypersil ODS 2,mobile phase composed of 0.1mol L -1 NaH 2PO 4 0.05mol L -1 Na 2SO 4 acetonitrile (36∶36∶28).The flow rate was 1.0ml.min -1 .The detective wavelength was 214nm. RESULTS:The high,medial and low average recovery and relative standard deviation was 99.55%,98.23% and 100.9%,respectively.RSD was 1.1%,1.7% and 0.61%,respectively. CONCLUSION:This method was rapid,simple and accurate.