HPLC法测定温六散中甘草酸单铵的含量

Determination of Glycyrrhizic acid in Wen liu San by HPLC

目的 :建立温六散中甘草酸单铵的含量测定方法。方法 :超声提取 ,使用 C1 8柱 ,甲醇 -含 3%冰醋酸的 0 .2 m ol/ L醋酸铵溶液 (6 2∶ 38)为流动相 ,检测波长为 2 5 0 nm。结果 :此方法线性关系良好 ,平均加样回收率为 99.5 % ,RSD为 1.8%。结论 :本方法分离良好 。

OBJECTIVE:To establish a method for the determination of the content of Glycyrrhizic acid in Wen Liu San.METHOD:Ultrasonic extraction,HPLC was used to determine Glycyrrhizic acid in Wen Liu San on C 18 Column with methanol 0.2 mol/LNH 4C 2H 3O 2 include 3% CH 3COOH(62∶38) mixture as mobile phase.The detection was at 250 nm.RESULTS:The linearity of this method was well.The average recovery was 99.5%, and RSD was 1.8%.CONCLUSION:This method has good peak resolution and can be used for quality control of Wen Liu San.