人类补体衰变加速因子对小鼠细胞保护作用的研究

Protective effect of human decay accelerating factor on mouse cells

目的 观察人类补体衰变加速因子 (DAF)在小鼠细胞表达后 ,对小鼠细胞抗人血清补体破坏的保护作用。方法 用人DAFcDNA转染小鼠细胞使该基因在小鼠细胞中表达。再将此种小鼠细胞放入含正常人血清的培养基中孵育 ,观察小鼠细胞是否被破坏。结果 转染人DAFcDNA并表达人DAF的小鼠细胞 ,在含人正常血清的培养基中孵育 48h后仍能存活并可以传代 ,而对照组的小鼠细胞孵育后在 1～ 4h内迅速死亡。结论 人类的DAF可以保护小鼠细胞不受补体的破坏。

Objective To observe the protective effect of human decay accelerating factor (DAF) expressed in mouse myeloma cells against the injury of human serum complement. Methods Mouse myeloma cells were transfected with human DAF cDNA and then put into normal human serum culture to obsetrve if they were injured. Results The mouse cells containing human DAF cDNA and expressing human DAF survived for more than 48 hours and passed on from generation to generation in the culture medium containing human normal serum. However, the control mouse cells died in 1～4 hours. Conclusion Human DAF protects mouse cells from injuring by human complement.