摘要
【目的】研究病毒性心肌炎 (VMC)心肌组织相容性抗原Ⅱ类 (MHCⅡ类抗原 )的表达特点 ,探索轻度、不典型VMC的法医病理学诊断方法。【方法】以 10 3 .5TCID50 的CoxsackieB3 病毒接种Balb/c小鼠 ,诱导小鼠轻度VMC。用免疫组化技术检测VMC小鼠心肌MHCⅡ类抗原的表达。【结果】VMC小鼠心肌组织内MHCⅡ类抗原异常表达 ,部分心肌细胞膜局灶性MHCⅡ类抗原阳性。【结论】心肌MHCⅡ类抗原的异常表达参与了轻度VMC的发病机制 ;心肌MHCⅡ类抗原 LSAB染色可望成为诊断轻度、不典型VMC的免疫病理学指标。
To investigate the expression of histocompatibility antigens class Ⅱ (MHC Ⅱ antigen) on myocardium in acute viral myocarditis(VMC) and study the diagnostic method for mild atypical VMC in the field of forensic pathology. Mild VMC model was induced in Balb/c mice by Coxsackie virus B 3. The hearts were studied LSAB immunohistochemically with anti MHC Ⅱ antibody. Aberrant expression of MHC Ⅱ antigen was found in the myocardium of mice with VMC. Focal sarcolemmal staining for MHC Ⅱ antigen was observed in some cardiomyocytes. [Conclusion] Aberrant expression of MHC Ⅱ antigen in myocardium is involved in the pathogenesis of acute mild VMC. MHC Ⅱ antigen LSAB immunohistochemical staining is expected to be an immunopathological index for diagnosing mild atypical VMC.
出处
《中山医科大学学报》
CSCD
北大核心
2001年第6期431-432,共2页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广东省自然科学基金 ( 95 0 30 1)
"2 11工程"重点学科建设课题
